Media & reagents : Consulting | |
Blood agar plate (BA) Use : For the isolation, cultivation and detection of hemolytic activity of streptococci, pneumococci and other particular fastidious microorganisms. Control organisms : Streptococcus pneumoniae : Good growth, Alpha - hemolysis Streptococcus pyogenes : Good growth, Beta-hemolysis Incubate: aerobic 35° C, 24 hrs. Shelf life : 4 weeks Price (Baht): 12 (return plate) , 15 (disposable plate) | |
Chocolate agar plate (CA) Use : For the isolation and cultivation of a variety of fastidious microorganism. Control organisms : Hemophilus influenzae and Neisseria gonorrhoeae : Good growth Incubate : CO2 ,35° C ,24 hrs. Shelf life : 4 weeks Price (Baht): 12 (return plate) , 15 (disposable plate) | |
MacConkey agar plate (MC) Use : For the selective isolation, cultivation and differentiation of coliformsand enteric pathogens based on the ability to ferment lactose. Lactose–fermening organisms appear as red to pink colonies. Lactose-nonfermenting organisms appear as colorless or transparent colonies Control organisms : Escherichia coli : Pink colonies (lactose fermentation) Proteus mirabilis : Colorless colonies ,no spreading Enterococcus sp. : No growth Incubate : Aerobic, 35° C, 24 hrs. Shelf life : 4 weeks Price (Baht): 12 (return plate) , 15 (disposable plate) | |
Mueller-Hinton agar plate (MHA) Use : For antimicrobial susceptibility testing of a variety of nonfastidious, rapidly-growing microorganisms. Control organisms : Escherichia coli ATCC25922 , Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853 : Growth ,Inhibition Zone followed Standard interpretative Incubate : Aerobic 35° C, 24 hrs Shelf life : 4 weeks Price (Baht): 12 (return plate) , 15 (disposable plate) | |
Salmonella-Shigella agar plate (SS) Use : For the selective isolation and differentiation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella. This media is not recommended for the primary isolation of Shigella species. Lactose-fermenting bacteria such as Escherichia coli or Klebsiella pneumoniae appear as small pink or red colonies. Lactose-nonfermenting bacteria such as Salmonella species, Proteus species and Shigella species appear as colorless colonies. Production of H2S by Salmonella species turns the center the colonies black. Control organisms : Salmonella typhi : Colorless colonies, black centers. Escherichia coli : Pink colonies Incubate : Aerobic 35° C, 24 hrs Shelf life : 4 weeks Price (Baht): 12 (return plate) , 15 (disposable plate) | |
Eosin Methylene Blue Agar (EMB) Use : For the isolation, cultivation and differentiation of Gram-negative enteric bacteria based on lactose fermentation. Bacteria that ferment lactose, especially the coliform bacterium Escherichia coli, Appear as colonies with green metallic sheen or blue–black to brown color. Bacteria that do not ferment lactose appear as colorless or transparent light purple colonies Control organisms : Escherichia coli : Good growth, green metallic sheen. Klebsiella pneumoniae : Good growth, purple colonies, no sheen. Shigella flexneri : Good growth, transparent colonies (lactose negative) Incubate : Aerobic 35° C, 24 hrs Shelf life : 4 weeks Price (Baht): 12 (return plate) , 15 (disposable plate) | |
Thiosulfate Citrate Bile Salt Sucrose agar plate (TCBS) Use : For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from a variety of clinical specimens and in epidemiological investigations. Control organisms: Vibrio cholerae : Growth (yellow colonies) Vibrio parahaemolyticus : Growth (green colonies) Staphylococcus aureus : No growth Incubate : Aerobic 35° C, 24 hrs Shelf life : 4 weeks Price (Baht): 15 (return plate) , 18 (disposable plate) | |
Sabouraud Dextrose Agar plate (SDA) Use : For the cultivation of pathogenic and nonpathogenic fungi, especially dermatophytes. The medium may be made more selective for fungi by the addition of specific antibiotics such as chloramphenicol. For the cultivation of yeast and filamentous fungi. Control organisms : Aspergillus flavus, Candida albicans : Growth Streptococcus pneumoniae : No growth Incubate : Aerobic 35° C, 24 hrs Shelf life : 4 weeks Price (Baht): 15 (return plate) , 18 (disposable plate) | |
Trypticase soy broth for Hemoculture Use : For the cultivation of a wide variety of fastidious and nonfastidious microorganisms from blood specimen and in epidemiological investigations. Also used for rapid estimation of the bacteriological quality of water. Control organisms: Staphylococcus aureus : Good growth Uninoculated tube : No growth and clear Incubate : 5% CO2, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 10 for 45 ml of TSB (return bottle) , 8 for 27 ml of TSB (return bottle) | |
Triple Sugar Iron agar slant (TSI slant) Use : For the differentiation of members of the Enterobacteriaceae base on their fermentation of lactose, sucrose and glucose and the production of H2S. Control organisms : Escherichia coli : A/A (G) Shigella flexneri : K/A Proteus vugaris : K/A, H2S Pseudomonas aeruginosa : K/N Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Motility Indole Lysine Medium (MIL slant) Use : For the cultivation and differentiation of members of the Enterobacteriaceae on the basis of motility, lysine decarboxylase activity, lysine deaminase activity and indole production. Control organisms : Proteus vulgaris : Motile / Indole/ LDC/ LDM ; +/+/-/+ Klebseilla pneumoniae : Motile / Indole/ LDC/ LDM ; -/-/+/- Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Christensen Urea Agar slant Use : For the differentiation of a variety of microorganisms, especially members of the Enterobacteriaceae, aerobic actinomycetes, streptococci and nonfermenting Gram-negative bacteria, on the basis of urease production. Control organisms : Proteus mirabilis : Pink throughout (positive) Klebsiella pneumoniae : Pink slant (partial positive) Escherichia coli : Yellow (negative) Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Simmons’ Citrate Agar slant Use : For the differentiation of Gram-negative bacteria on the basis of citrate utilization. Bacteria which can utilize citrate as sole carbon source turn medium blue. Control organisms : Klebsiella pneumoniae : Growth and blue color (positive) Escherichia coli : No growth, remains green (negative) Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Bile Esculin Agar slant Use : For the isolation and presumptive identification of enterococci (Group D streptococci). Control organisms : Enterococcus spp. : Good growth, black Alpha-Hemolytic Streptococcus, not group D : No growth, colorless on media Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
6.5% NaCl Use : For for cultivation and differentiation of bacteria based on their ability to growth on 6.5% NaCl tolerance. Control organisms : Enterococcus faecalis : Yellow (positive) Group D nonenterococci: Remains orange (negative) Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Pigment medium Use : For the cultivation and differentiation of Streptococcus agalactiae (Group B Streptococcus) Control organisms : Streptococcus agalactiae : Orange pigment in stabbing (positive) Beta-streptococcus not gr. A, B or D : Remains colorless (negative) Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Ornithine Decarboxylase slant (ODC) Use : For the cultivation and differentiation of bacteria based on their ability to decarboxylate the amino acid. Bacteria that decaboxylate ornithine turn the medium turbid purple. Control organisms : Proteus mirabilis : Purple (positive) Klebsiella pneumoniae : Yellow (negative) Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Selenite-F Broth (SF) Use : For the isolation and cultivation of Salmonella species from feces and other specimens. Control organisms : Salmonella spp. : Growth Escherichai coli : No growth Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Thioglycollate Medium Use : For the cultivation of both aerobic and anaerobic organisms in the performance of sterility test. Control organisms : Staphylococcus aureus : aerobic growth Clostridium spp. : anaerobic growth Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 10 (return tube) | |
Trypicase Soy Broth (TSB) Use : For the cultivation of a wide variety of fastidious and nonfastidious microorganisms from clinical specimen and in epidemiological investigations. Also used for rapid estimation of the bacteriological quality of water. Control organisms : Staphylococcus aureus : Good growth Uninoculated tube : No growth and clear Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Phenol Red Glucose (PR Glucose) Use : For the determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid –medium turns yellow. Control organisms : Staphylococcus aureus : Growth (yellow) Micrococcus spp. : Growth (remain orange) Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Phenol Red Mannitol (PR Mannitol) Use : For the determination of the ability of a microorganism to ferment manitol. Fermentation is determined by the production of acid –medium turns yellow. Control organisms : Staphylococcus aureus : Growth (yellow) Staphylococcus epidermidis : Growth (remain orange) Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Phenol Red Sucrose (PR Sucrose) Use : For the determination of the ability of a microorganism to ferment sucrose. Fermentation is determined by the production of acid –medium turns yellow. Control organisms : Vibrio cholerae : Growth (yellow) Vibrio parahaemolyticus : Growth (remain orange) Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Phenol Red Arabinose (PR Arabinose) Use : For the determination of the ability of a microorganism to ferment arabinose. Fermentation is determined by the production of acid –medium turns yellow. Control organisms : Enterococcus faecium : Growth (yellow) Enterococcus faecalis : Growth (remain orange) Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Phenol Red Lactose (PR Lactose) Use : For the determination of the ability of a microorganism to ferment lactose. Fermentation is determined by the production of acid –medium turns yellow. Control organisms : Incubate : Aerobic, 35° C , 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Oxidative Fermentation Glucose (OF Glucose ) Use : For differentiating Gram-negative bacteria based upon determining the oxidative and fermentative metabolism of glucose. Bacteria that ferment glucose turn the medium yellow. Control organisms : Pseudomonas aeruginosa : Yellow (positive) Acinetobacter lwoffii : Remains green Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Oxidation Fermentation Maltose (OF Maltose) Use : For differentiating Gram-negative bacteria based upon determining the oxidative and fermentative metabolism of maltose. Bacteria that ferment glucose turn the medium yellow. Control organisms : Stenotrophomonas maltophilia : Yellow Pseudomonas aeruginosa : Remains green Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Lowenstein-Jensen Medium Use : For the cultivation and differentiation of Mycobacterium species. M.tuberculosis appears as granular ,rough, dry colonies. M.kansasii appears as smooth to rough photochromogenic colonies. M.gordonae appears as smooth yellow- orange colonies. M.avium appears as smooth, colorless colonies. M.smegmatis appears as wrinkled, creamy white colonies. Also used for the cultivation and maintenance of Gordona species, Nocardia species, Rhodococcus species, and Tsukamurella paurometabolum. Control organisms : Mycobacterium phlei : Good growth Uninoculated slant : No growth Incubate : Aerobic 35° C, 3 days Shelf life : 4 weeks Price (Baht): 10 (return tube) | |
Stuart Transport medium Use : For transport of swabs of clinical material containing upper respiratorytract, enteric pathogens and other fastidious organisms. Control organisms : Streptococcus pyogenes : Growth Uninoculated medium : No growth Incubate : Aerobic 35° C, 24 hrs Shelf life : 8 weeks Price (Baht): 5 (return tube) | |
Kovac's reagent Use : For determination of bacteria that posses the enzyme tryptophanase are capable of cleaving tryptophan, thereby producing indole, pyruvic acid and ammonia. Indole can be detected in tryptophan medium by observing the development of red color after adding a solution containing p-dimethylamino-benzaldehyde. (Kovac's reagent) Control organisms : Escherichia coli : Red (Indole positive) Klebsiella sp. : remained yellow (Indole negative) Shelf life : 1 year Price (Baht): 400 per 100 ml | |
Gram's stain Use : For differentiating Gram-positive and negative bacteria. Control organisms : Escherichia coli : red cell (Gram negative) Staphylococcus aureus : dark purple cell (Gram positive) Shelf life : 1 year Price (Baht): 400 per set (Crystal violet, Gram's iodine, Decolorizer and Safranin ; each 100 ml) | |
Acid fast stain Use : For the determination of the Mycobacterium, Nocardia, and Corynebacterium Control organisms : Mycobacterium tubercurosis : red cell Escherichia coli : blue cell Shelf life : 1 year Price (Baht): 500 per set (Carbon fuchsin, 3% Acid alcohol and Methylene blue ;each 100 ml) Carbon fuchsin only 3 per ml. | |
3 مشترك
Media reagents
العبادل- طالب متميز
عدد الرسائل : 257
العمر : 37
البلد : الـــيـــمـــن
القسم والمستوى : علوم طبيه قسم مخـــتــبــرات
المزاج : شــــاعــــر
:
السٌّمعَة : 0
نقاط : 372
تاريخ التسجيل : 12/03/2009
بطاقة الشخصية
تخصصي: طب
المحافظة: حجة
عمر النجاده- مراقب عام المنتدى
عدد الرسائل : 630
العمر : 36
البلد : اليمن
القسم والمستوى : خريج / مختبرات طبــــيـــــه
المزاج : طبيعــــــي
العضوية : 107
أختر علم دولتك :
:
السٌّمعَة : 0
نقاط : 202
تاريخ التسجيل : 08/03/2008
بطاقة الشخصية
تخصصي: طب
المحافظة: الحديدة
يعطيـــــــــــك اخي الغالي الف الف الف عافيــــــــــــه
موضووووووووعك رووووووووعه
الان لازم كل طلاب المختبرات اللي مو عارفين اسماء الأطباق واستخداماتها ان يدخلوا على هذه الصفحه
تعجز كلماتي عن شكرك اخي الغالي
دمت متألقا دوما
موضووووووووعك رووووووووعه
الان لازم كل طلاب المختبرات اللي مو عارفين اسماء الأطباق واستخداماتها ان يدخلوا على هذه الصفحه
تعجز كلماتي عن شكرك اخي الغالي
دمت متألقا دوما
مأمون المفلحي- مستشار إداري
عدد الرسائل : 2676
العمر : 37
البلد : في كل حلم جميل
القسم والمستوى : برمجة حاسوب
المزاج : أحب الهــــدووووووووء والصراحة
:
السٌّمعَة : 13
نقاط : 1666
تاريخ التسجيل : 26/04/2008
تسلم أخ العبادل ع المجهود الجميل
ونتمنى من كل طلاب المختبرات الإستفادة من هذا المجهود الجميل..؟
تحياتي لك أخي العزيز
ونتمنى من كل طلاب المختبرات الإستفادة من هذا المجهود الجميل..؟
تحياتي لك أخي العزيز
العبادل- طالب متميز
عدد الرسائل : 257
العمر : 37
البلد : الـــيـــمـــن
القسم والمستوى : علوم طبيه قسم مخـــتــبــرات
المزاج : شــــاعــــر
:
السٌّمعَة : 0
نقاط : 372
تاريخ التسجيل : 12/03/2009
بطاقة الشخصية
تخصصي: طب
المحافظة: حجة
أتشرف بمرور شخصك الكريم أخي وأستاذي / مأمون المفلحي
مع خالص الشكر والتقدير