منتدى طلاب جامعة الحديدة

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منتدى طلاب جامعة الحديدة


    Media reagents

    شاطر

    العبادل
    طالب متميز
    طالب متميز

    ذكر
    عدد الرسائل : 257
    العمر : 29
    البلد : الـــيـــمـــن
    القسم والمستوى : علوم طبيه قسم مخـــتــبــرات
    المزاج : شــــاعــــر
      :
    السٌّمعَة : 0
    نقاط : 372
    تاريخ التسجيل : 12/03/2009

    بطاقة الشخصية
    تخصصي: طب
    المحافظة: حجة

    Media reagents

    مُساهمة من طرف العبادل في السبت فبراير 06, 2010 7:13 pm


    Media & reagents : Consulting



    Blood agar plate (BA)

    Use : For the isolation, cultivation and detection of hemolytic activity of streptococci, pneumococci and other particular fastidious microorganisms.

    Control organisms :
    Streptococcus pneumoniae
    : Good growth, Alpha - hemolysis Streptococcus pyogenes : Good growth, Beta-hemolysis

    Incubate: aerobic 35° C, 24 hrs.
    Shelf life
    : 4 weeks
    Price (Baht)
    : 12 (return plate) , 15 (disposable plate)


    Chocolate agar plate (CA)

    Use : For the isolation and cultivation of a variety of fastidious microorganism.

    Control organisms :
    Hemophilus influenzae and Neisseria gonorrhoeae : Good growth

    Incubate : CO2 ,35° C ,24 hrs.
    Shelf life
    : 4 weeks
    Price (Baht)
    : 12 (return plate) , 15 (disposable plate)



    MacConkey agar plate (MC)

    Use : For the selective isolation, cultivation and differentiation of coliformsand enteric pathogens based on the ability to ferment lactose. Lactose–fermening organisms appear as red to pink colonies. Lactose-nonfermenting organisms appear as colorless or transparent colonies

    Control organisms :
    Escherichia coli : Pink colonies (lactose fermentation)
    Proteus mirabilis : Colorless colonies ,no spreading
    Enterococcus sp. : No growth

    Incubate : Aerobic, 35° C, 24 hrs.
    Shelf life : 4 weeks
    Price (Baht)
    : 12 (return plate) , 15 (disposable plate)


    Mueller-Hinton agar plate (MHA)

    Use : For antimicrobial susceptibility testing of a variety of nonfastidious, rapidly-growing microorganisms.

    Control organisms :
    Escherichia coli ATCC25922 , Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853 : Growth ,Inhibition Zone followed Standard interpretative

    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 4 weeks
    Price (Baht)
    : 12 (return plate) , 15 (disposable plate)


    Salmonella-Shigella agar plate (SS)

    Use : For the selective isolation and differentiation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella. This media is not recommended for the primary isolation of Shigella species. Lactose-fermenting bacteria such as Escherichia coli or Klebsiella pneumoniae appear as small pink or red colonies. Lactose-nonfermenting bacteria such as Salmonella species, Proteus species and Shigella species appear as colorless colonies. Production of H2S by Salmonella species turns the center the colonies black.

    Control organisms :
    Salmonella typhi : Colorless colonies, black centers. Escherichia coli : Pink colonies

    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 4 weeks
    Price (Baht)
    : 12 (return plate) , 15 (disposable plate)


    Eosin Methylene Blue Agar (EMB)

    Use : For the isolation, cultivation and differentiation of Gram-negative enteric bacteria based on lactose fermentation. Bacteria that ferment lactose, especially the coliform bacterium Escherichia coli, Appear as colonies with green metallic sheen or blue–black to brown color. Bacteria that do not ferment lactose appear as colorless or transparent light purple colonies

    Control organisms :
    Escherichia coli : Good growth, green metallic sheen.
    Klebsiella pneumoniae : Good growth, purple colonies, no sheen.
    Shigella flexneri : Good growth, transparent colonies (lactose negative)


    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 4 weeks
    Price (Baht)
    : 12 (return plate) , 15 (disposable plate)


    Thiosulfate Citrate Bile Salt Sucrose agar plate (TCBS)

    Use : For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from a variety of clinical specimens and in epidemiological investigations.

    Control organisms:
    Vibrio cholerae : Growth (yellow colonies)
    Vibrio parahaemolyticus : Growth (green colonies)
    Staphylococcus aureus : No growth

    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 4 weeks
    Price (Baht)
    : 15 (return plate) , 18 (disposable plate)


    Sabouraud Dextrose Agar plate (SDA)

    Use : For the cultivation of pathogenic and nonpathogenic fungi, especially dermatophytes. The medium may be made more selective for fungi by the addition of specific antibiotics such as chloramphenicol. For the cultivation of yeast and filamentous fungi.

    Control organisms :
    Aspergillus flavus, Candida albicans : Growth
    Streptococcus pneumoniae : No growth

    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 4 weeks
    Price (Baht)
    : 15 (return plate) , 18 (disposable plate)


    Trypticase soy broth for Hemoculture

    Use : For the cultivation of a wide variety of fastidious and nonfastidious microorganisms from blood specimen and in epidemiological investigations. Also used for rapid estimation of the bacteriological quality of water.

    Control organisms:
    Staphylococcus aureus : Good growth
    Uninoculated tube : No growth and clear


    Incubate : 5% CO2, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 10 for 45 ml of TSB (return bottle) , 8 for 27 ml of TSB (return bottle)


    Triple Sugar Iron agar slant (TSI slant)

    Use : For the differentiation of members of the Enterobacteriaceae base on their fermentation of lactose, sucrose and glucose and the production of H2S.

    Control organisms :
    Escherichia coli : A/A (G)
    Shigella flexneri : K/A
    Proteus vugaris : K/A, H2S
    Pseudomonas aeruginosa
    : K/N

    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Motility Indole Lysine Medium (MIL slant)

    Use : For the cultivation and differentiation of members of the Enterobacteriaceae on the basis of motility, lysine decarboxylase activity, lysine deaminase activity and indole production.

    Control organisms :
    Proteus vulgaris
    : Motile / Indole/ LDC/ LDM ; +/+/-/+
    Klebseilla pneumoniae
    : Motile / Indole/ LDC/ LDM ; -/-/+/-


    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Christensen Urea Agar slant

    Use : For the differentiation of a variety of microorganisms, especially members of the Enterobacteriaceae, aerobic actinomycetes, streptococci and nonfermenting Gram-negative bacteria, on the basis of urease production.

    Control organisms :
    Proteus mirabilis
    : Pink throughout (positive)
    Klebsiella pneumoniae : Pink slant (partial positive)
    Escherichia coli : Yellow (negative)


    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Simmons’ Citrate Agar slant

    Use : For the differentiation of Gram-negative bacteria on the basis of citrate utilization. Bacteria which can utilize citrate as sole carbon source turn medium blue.

    Control organisms :
    Klebsiella pneumoniae
    : Growth and blue color (positive)
    Escherichia coli
    : No growth, remains green (negative)


    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Bile Esculin Agar slant

    Use : For the isolation and presumptive identification of enterococci (Group D streptococci).

    Control organisms :
    Enterococcus
    spp. : Good growth, black
    Alpha-Hemolytic Streptococcus, not group D
    : No growth, colorless on media

    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    6.5% NaCl

    Use : For for cultivation and differentiation of bacteria based on their ability to growth on 6.5% NaCl tolerance.

    Control organisms :
    Enterococcus faecalis : Yellow (positive)
    Group D nonenterococci: Remains orange (negative)


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Pigment medium

    Use : For the cultivation and differentiation of Streptococcus agalactiae (Group B Streptococcus)

    Control organisms :
    Streptococcus agalactiae : Orange pigment in stabbing (positive)
    Beta-streptococcus not gr. A, B or D : Remains colorless (negative)


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Ornithine Decarboxylase slant (ODC)

    Use : For the cultivation and differentiation of bacteria based on their ability to decarboxylate the amino acid. Bacteria that decaboxylate ornithine turn the medium turbid purple.

    Control organisms :
    Proteus mirabilis : Purple (positive)
    Klebsiella pneumoniae : Yellow (negative)


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Selenite-F Broth (SF)

    Use : For the isolation and cultivation of Salmonella species from feces and other specimens.

    Control organisms :
    Salmonella spp. : Growth
    Escherichai coli : No growth


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)

    Thioglycollate Medium

    Use : For the cultivation of both aerobic and anaerobic organisms in the performance of sterility test.

    Control organisms :
    Staphylococcus aureus : aerobic growth
    Clostridium spp. : anaerobic growth


    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 10 (return tube)

    Trypicase Soy Broth (TSB)

    Use : For the cultivation of a wide variety of fastidious and nonfastidious microorganisms from clinical specimen and in epidemiological investigations. Also used for rapid estimation of the bacteriological quality of water.

    Control organisms :
    Staphylococcus aureus
    : Good growth
    Uninoculated tube : No growth and clear


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Phenol Red Glucose (PR Glucose)

    Use : For the determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid –medium turns yellow.

    Control organisms :
    Staphylococcus aureus
    : Growth (yellow)
    Micrococcus spp. : Growth (remain orange)


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)

    Phenol Red Mannitol (PR Mannitol)

    Use : For the determination of the ability of a microorganism to ferment manitol. Fermentation is determined by the production of acid –medium turns yellow.

    Control organisms :
    Staphylococcus aureus : Growth (yellow)
    Staphylococcus epidermidis : Growth (remain orange)


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)

    Phenol Red Sucrose (PR Sucrose)

    Use : For the determination of the ability of a microorganism to ferment sucrose. Fermentation is determined by the production of acid –medium turns yellow.

    Control organisms :
    Vibrio cholerae
    : Growth (yellow)
    Vibrio parahaemolyticus
    : Growth (remain orange)


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)

    Phenol Red Arabinose (PR Arabinose)

    Use : For the determination of the ability of a microorganism to ferment arabinose. Fermentation is determined by the production of acid –medium turns yellow.

    Control organisms :
    Enterococcus faecium
    : Growth (yellow)
    Enterococcus faecalis : Growth (remain orange)


    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)

    Phenol Red Lactose (PR Lactose)

    Use : For the determination of the ability of a microorganism to ferment lactose. Fermentation is determined by the production of acid –medium turns yellow.

    Control organisms :

    Incubate : Aerobic, 35° C , 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Oxidative Fermentation Glucose (OF Glucose )

    Use : For differentiating Gram-negative bacteria based upon determining the oxidative and fermentative metabolism of glucose. Bacteria that ferment glucose turn the medium yellow.

    Control organisms :
    Pseudomonas aeruginosa
    : Yellow (positive)
    Acinetobacter lwoffii
    : Remains green

    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)

    Oxidation Fermentation Maltose (OF Maltose)

    Use : For differentiating Gram-negative bacteria based upon determining the oxidative and fermentative metabolism of maltose. Bacteria that ferment glucose turn the medium yellow.

    Control organisms :
    Stenotrophomonas maltophilia
    : Yellow
    Pseudomonas aeruginosa
    : Remains green


    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Lowenstein-Jensen Medium

    Use : For the cultivation and differentiation of Mycobacterium species. M.tuberculosis appears as granular ,rough, dry colonies. M.kansasii appears as smooth to rough photochromogenic colonies. M.gordonae appears as smooth yellow- orange colonies. M.avium appears as smooth, colorless colonies. M.smegmatis appears as wrinkled, creamy white colonies. Also used for the cultivation and maintenance of Gordona species, Nocardia species, Rhodococcus species, and Tsukamurella paurometabolum.

    Control organisms :
    Mycobacterium phlei
    : Good growth
    Uninoculated slant : No growth


    Incubate : Aerobic 35° C, 3 days
    Shelf life
    : 4 weeks
    Price (Baht)
    : 10 (return tube)


    Stuart Transport medium

    Use : For transport of swabs of clinical material containing upper respiratorytract, enteric pathogens and other fastidious organisms.

    Control organisms :
    Streptococcus pyogenes
    : Growth
    Uninoculated medium : No growth


    Incubate : Aerobic 35° C, 24 hrs
    Shelf life
    : 8 weeks
    Price (Baht)
    : 5 (return tube)


    Kovac's reagent

    Use : For determination of bacteria that posses the enzyme tryptophanase are capable of cleaving tryptophan, thereby producing indole, pyruvic acid and ammonia. Indole can be detected in tryptophan medium by observing the development of red color after adding a solution containing p-dimethylamino-benzaldehyde. (Kovac's reagent)

    Control organisms :
    Escherichia coli
    : Red (Indole positive)
    Klebsiella sp. : remained yellow (Indole negative)


    Shelf life : 1 year
    Price (Baht)
    : 400 per 100 ml


    Gram's stain

    Use : For differentiating Gram-positive and negative bacteria.

    Control organisms :
    Escherichia coli : red cell (Gram negative)
    Staphylococcus aureus : dark purple cell (Gram positive)


    Shelf life : 1 year
    Price (Baht)
    : 400 per set (Crystal violet, Gram's iodine, Decolorizer and Safranin ; each 100 ml)


    Acid fast stain

    Use : For the determination of the Mycobacterium, Nocardia, and Corynebacterium

    Control organisms :
    Mycobacterium tubercurosis : red cell
    Escherichia coli : blue cell


    Shelf life : 1 year
    Price (Baht)
    : 500 per set (Carbon fuchsin, 3% Acid alcohol and Methylene blue ;each 100 ml) Carbon fuchsin only 3 per ml.




    عمر النجاده
    مراقب عام المنتدى
    مراقب عام المنتدى

    ذكر
    عدد الرسائل : 630
    العمر : 29
    البلد : اليمن
    القسم والمستوى : خريج / مختبرات طبــــيـــــه
    المزاج : طبيعــــــي
    العضوية : 107
    أختر علم دولتك :
      :
    السٌّمعَة : 0
    نقاط : 202
    تاريخ التسجيل : 08/03/2008

    بطاقة الشخصية
    تخصصي: طب
    المحافظة: الحديدة

    رد: Media reagents

    مُساهمة من طرف عمر النجاده في الجمعة فبراير 19, 2010 9:49 am

    يعطيـــــــــــك اخي الغالي الف الف الف عافيــــــــــــه
    موضووووووووعك رووووووووعه

    الان لازم كل طلاب المختبرات اللي مو عارفين اسماء الأطباق واستخداماتها ان يدخلوا على هذه الصفحه

    تعجز كلماتي عن شكرك اخي الغالي
    دمت متألقا دوما


    _________________

    مأمون المفلحي
    مستشار إداري
    مستشار إداري

    ذكر
    عدد الرسائل : 2675
    العمر : 29
    البلد : في كل حلم جميل
    القسم والمستوى : برمجة حاسوب
    المزاج : أحب الهــــدووووووووء والصراحة
      :
    السٌّمعَة : 13
    نقاط : 1663
    تاريخ التسجيل : 26/04/2008

    رد: Media reagents

    مُساهمة من طرف مأمون المفلحي في السبت فبراير 20, 2010 11:50 am

    تسلم أخ العبادل ع المجهود الجميل
    ونتمنى من كل طلاب المختبرات الإستفادة من هذا المجهود الجميل..؟
    تحياتي لك أخي العزيز



    _________________

    الحيـــــــــــــــــــــاة دمعـتان .. دمعــة لقاء ودمعــة وداع .. والأصعب من ذلك دمعة لقاء بعد الفــــــــــــراق...!!!

    العبادل
    طالب متميز
    طالب متميز

    ذكر
    عدد الرسائل : 257
    العمر : 29
    البلد : الـــيـــمـــن
    القسم والمستوى : علوم طبيه قسم مخـــتــبــرات
    المزاج : شــــاعــــر
      :
    السٌّمعَة : 0
    نقاط : 372
    تاريخ التسجيل : 12/03/2009

    بطاقة الشخصية
    تخصصي: طب
    المحافظة: حجة

    رد: Media reagents

    مُساهمة من طرف العبادل في السبت فبراير 20, 2010 7:02 pm



    أتشرف بمرور شخصك الكريم أخي وأستاذي / مأمون المفلحي
    مع خالص الشكر والتقدير

      الوقت/التاريخ الآن هو الأحد ديسمبر 04, 2016 11:28 pm